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. 2013 Sep 24;8(9):e75787. doi: 10.1371/journal.pone.0075787

Table 2. Antibodies used in immunohistochemistry.

Antibody (dilution) Isotype Pre-treatment Specificity Source/ Reference
1B5 (1:5) IgG1,kappa ABC C-0-S [62,63]
2B6 (1:5) IgG1,kappa ABC C-4-S/DS [62,63]
3B3 (1:5) IgM, kappa ABC C-6-S [63]
5D4 (1:5) IgG1,kappa ABC* KS [64]

ABC, chondroitinase ABC; C-0-S, chondroitin-0-sulfate; C-4-S, chondroitin-4-sulpfate; C-6-S, chondroitin-6-sulfate; DS, dermatan sulfate; KS, keratan sulphate; * chondroitinase ABC was used to remove CS chains for improved KS labelling.