Table 2. Antibodies used in immunohistochemistry.
Antibody (dilution) | Isotype | Pre-treatment | Specificity | Source/ Reference |
---|---|---|---|---|
1B5 (1:5) | IgG1,kappa | ABC | C-0-S | [62,63] |
2B6 (1:5) | IgG1,kappa | ABC | C-4-S/DS | [62,63] |
3B3 (1:5) | IgM, kappa | ABC | C-6-S | [63] |
5D4 (1:5) | IgG1,kappa | ABC* | KS | [64] |
ABC, chondroitinase ABC; C-0-S, chondroitin-0-sulfate; C-4-S, chondroitin-4-sulpfate; C-6-S, chondroitin-6-sulfate; DS, dermatan sulfate; KS, keratan sulphate; * chondroitinase ABC was used to remove CS chains for improved KS labelling.