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. 2013 Sep 24;8(9):e72169. doi: 10.1371/journal.pone.0072169

Figure 5. Aβ25–35 induces oxidative stress and DNA damage.

Figure 5

A) ROS produced by SH-SY5Y cells treated with 10 µM Aβ25–35 at different times were revealed by flow cytometry using DCF-DA (white bar  =  control cells; black bar  =  Aβ25–35 treated cells; fine dotted bar  =  cells treated with Aβ25–35 in the presence of quercetin). B) DNA damage was evaluated by Comet assay and expressed as Olive moment in SH-SY5Y treated with 10 µM Aβ25–35 for 24 and 48 h (white bar  =  control cells; black bar  =  Aβ25–35 treated cells). C) PARP-1 activity in SH-SY5Y treated with 10 µM Aβ25–35 in the presence or absence of 5 µM quercetin by the use of a dedicated colorimetric Kit from Trevigen (white bar  =  control cells; black bar  =  Aβ25–35 treated cells; fine dotted bar  =  cells treated with Aβ25–35 in the presence of quercetin). Results are the mean of at least three independent experiments ± S.D. *p<0.05 vs. control cells; § p<0.05 vs. Aβ25–35-treated cells.