Figure 5. Fluorescent sections from heart and liver show highly efficient cardiac knockdown and no liver knockdown.

Mice were injected with AAV9 capsids containing the single-stranded pAUSiGTL vector expressing shGFP or AAV9 containing an off-target shRNA. Tissues were harvested 7 weeks after injection. (A) and (E) show heart and liver, respectively, treated with the off-target shRNA (scale bars = 500 µm), while (C) and (G) show the same sections magnified in the area of the white boxes (scale bars = 50 µm). (B) and (F) show heart and liver, respectively, treated with shGFP (scale bars = 500 µm), while (D) and (H) show the same sections magnified in the area of the white box (scale bars = 50 µm).