Figure 1. Vaccination with Lm-LLO-E7 induces the expression of chemokines by TC-1 tumors. (A) cDNA from three mice that were treated twice with either Dulbecco’s PBS (DPBS) or Lm-LLO-E7 bacteria were pooled and applied to chemokine PCR arrays. Data are reported as fold changes in the expression of the indicated mRNA species in TC-1 tumors isolated from Lm-LLO-E7-treated mice relative to DPBS-treated animals. Data are from a single experiment conducted once. (B and C) Conventional quantitative RT-PCR assays were conducted on tumor RNA samples (n = 3) (B) and tumor lysates were analyzed using chemokine ELISAs in order to confirm the presence of vaccine-induced TH1 chemokines (CXCL9 and CXCL10, n = 8, pooled from 2 experiments; CCL4 and CCL5, n = 3) (C). Mean RQ (B) or protein concentration values (C) ± SEM are reported (*P≤ 0.05, ** P ≤ 0.01, ***P < 0.001). Conventional quantitative RT-PCR primer assays and ELISAs were conducted twice with similar results.