Figure 5. Knockdown of CXCL9 in TC-1 cells alters T-cell subset distribution in the tumor plugs of vaccinated mice. (A–C) TC-1 cells bearing a control plasmid or a shRNA plasmid for the downregulation of CXCL9 were co-injected with basement membrane extract (BME) and allowed to form plugs for 7 d. On day 7, mice were vaccinated with Lm-LLO-E7 bacteria and, 7 d later, tumor plugs were removed and processed for the cytofluorometric measurement of the percentages of CXCR3⁺TCRβ⁺ as well as CD8⁺CXCR3⁺TCRβ⁺ and CD4⁺CXCR3⁺TCRβ⁺ T lymphocytes (among live single cells). The percentage of CXCR3⁺TCRβ⁺ cells is reported in panel (A) whereas representative density plots of the relative abundance of CD8⁺CXCR3⁺TCRβ⁺ and CD4⁺CXCR3⁺TCRβ⁺ T lymphocytes is depicted in (B) In (C) quantitative data on the percentages of CD8⁺CXCR3⁺TCRβ⁺ and CD4⁺CXCR3⁺TCRβ⁺ T cells as well as on their ratio are reported. In (A and C) columns represent mean values ± SEM of pooled data (n = 7) from two independent experiments (2–5 mice per group) (*P ≤ 0.05, **P ≤ 0.01, ***P < 0.001).