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. 2013 Jul 11;8(3):731–736. doi: 10.3892/mmr.2013.1585

Figure 5.

Figure 5

Effect of HCT on PMA plus A23187-stimulated NF-κB activation and IκBα phosphorylation. HMC-1 cells (1×106 cells/ml) were incubated with HCT (0.05–0.2 mg/ml) for 1 h and stimulated with PMA plus A23187 for 2 h. Nuclear and cytoplasmic proteins were isolated by lysis buffer and examined for NF-κB and p-IκBα by western blot analysis. N, nuclear extract; C, cytosol extract; HCT, Houttuynia cordata Thunb.