Table 1. Transplant and MSC product characteristics and tissue GFP positivity in dogs conditioned with 920 cGy prior DLA-identical marrow transplantation with or without MSC infusion.
| |
|
|
MSC treatment |
|
|
||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Recipient ID (sex) | Donor ID | Marrow cells dose (TNC × 108/kg) |
MSC source | Infused (cells x 106) |
Viability (%) |
Viable MC dose (×106 cells/kg) |
Transduction efficiency (%) |
Transduced dose (106 cells/kg) |
GFP (+/−) |
Survival (days) |
|
| E645(F) |
E647 |
4.3 |
DLA identical (E647) |
67.7 |
96.2 |
7.4 |
18.0 |
1.35 |
+ |
43 |
|
| E647(F) |
E645 |
3.5 |
DLA identical (E645) |
68.9 |
93.8 |
7.5 |
21.4 |
1.57 |
- |
43 |
|
| E612(M) |
E613 |
3.1 |
DLA identical (E613) |
110 |
97.6 |
10 |
50.4 |
5.04 |
+ |
100 |
|
| E613(M) |
E612 |
2.0 |
DLA identical (E612) |
129 |
92.1 |
10 |
51.8 |
5.18 |
+ |
100 |
|
| |
|
|
|
|
|
|
|
|
|
|
|
| E705(F) |
E708 |
3.1 |
Unrelated (E450) |
116 |
87.6 |
10 |
|
|
+ |
9* |
|
| E708(M) |
E705 |
3.0 |
Unrelated (E450) |
102 |
86.1 |
10 |
|
|
- |
9* |
|
| E669(F) |
E672 |
4.0 |
Unrelated (E618) |
52.6 |
76.0 |
4.8 |
|
|
- |
43 |
|
| E672(M) |
E669 |
3.1 |
Unrelated (E618) |
82.8 |
77.4 |
5.1 |
|
|
+ |
43 |
|
| |
|
|
|
|
|
|
|
|
|
|
|
| E626(F) |
E628 |
5.3 |
NA |
NA |
NA |
NA |
NA |
NA |
NA |
104 |
|
| E628(M) |
E626 |
4.2 |
NA |
NA |
NA |
NA |
NA |
NA |
NA |
104 |
|
| E683(F) |
E684 |
3.0 |
NA |
NA |
NA |
NA |
NA |
NA |
NA |
98 |
|
| E684(M) |
E683 |
1.8 |
NA |
NA |
NA |
NA |
NA |
NA |
NA |
98 |
|
| E709(M) |
E706 |
2.0 |
NA |
NA |
NA |
NA |
NA |
NA |
NA |
102 |
|
| E706(F) | E709 | 2.7 | NA | NA | NA | NA | NA | NA | NA | 102 | |
Dogs were conditioned with 920 cGy total body irradiation followed by bone marrow transplantation and immunosuppression with cyclosporine (20 mg/kg twice daily from day 0 through 5 and 10 mg/kg from day 6 through the end of study). Mesenchymal stromal cells (MSC) were infused within 1 h of conditioning. *Euthanized on day 9 due to pulmonary edema (E705) and intussusception (E708). Tissue was collected at time of euthanasia and presence of green fluorescent protein (GFP) was detected by GFP specific PCR in bone marrow, spleen and liver (+, if GFP signal was positive in any of the three tested tissues; -, if GFP signal negative in all tissues tested). DLA, dog leukocyte antigen; TNC, total nucleated cells; NA, not applicable.