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. 2013 Sep 25;33(39):15350–15361. doi: 10.1523/JNEUROSCI.2510-13.2013

Figure 3.

Figure 3.

Regenerating axons are not spared axons. A, Serial sagittal sections through the lesion of AAV-shPTEN animal 550, stained for CST axons (BDA, white in single-channel images/red in color-merged images), GFAP (green), and nuclei (blue). B, Tracings of CST axons from A overlaid on top of each other to show that axons grow through the middle of the lesion and taper off after ∼2 mm. Color represents the left–right position of the axons. C, Cross sections of the spinal cord 3 mm rostral and 5 mm caudal to the lesion site (T8) of an AAV-shPTEN animal and an uninjured control, stained for CST axons (BDA). D, Sagittal sections through the lesion of AAV-shPTEN animals at 2, 3, and 4 weeks after injury, stained for CST axons (BDA, white in single-channel images/red in color-merged images), GFAP (green), and nuclei (blue). Arrows indicate axons at the edge of and beyond the lesion at 3 and 4 weeks, respectively. E, Quantification of axon regeneration at various distances from the distal edge of the lesion normalized to the number of axons labeled in the brainstem pyramidal tract for AAV-shPTEN-injected animals perfused at 2, 3, and 4 weeks after injury. Error bars indicate SEM. R, Rostral; C, caudal; D, dorsal; V, ventral. Scale bars: A–C, 500 μm; D, 250 μm.