Figure 5.

Basal Akt and ERK1/2 signaling in adult Dab1 cKO mice. Western blot analysis of the phosphorylation status of Akt and ERK1/2 in adult WT and Dab1 cKO cortex (CX) and hippocampus (HC). The data were quantified as the ratio of phospho-Akt (p-Akt) over total Akt, and phospho-ERK2 (p-ERK2) over total ERK2. There were no significant differences in the levels of p-Akt and p-ERK1/2 between genotypes at 2 months of age (A). However, at 4 months of age (B) the levels of p-Akt and p-ERK2 were significantly reduced in both the cortex and hippocampus of Dab1 cKO compared with WT mice (n = 4 mice per genotype). Bar graphs indicate the mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001.