Fig. 5.

Migration of neuroblasts in the RMS after ischaemia. Coronal sections from the anterior forebrain of normal (A) and post-ischaemic (B, C) rats were immunostained for Dcx. At these levels, Dcx + cells are observed only within the RMS. The number of migrating Dcx + cells remained normal at the sub-acute phase post-injury (compare A with B), but was decreased at 1 year post-ischaemia (C). (D) Graph showing the quantification of cells in the RMS in sham-operated and post-ischaemic rats. Note that the Dcx negative fraction of cells (possibly the structural components of the stream) is not affected by ischaemia. (Panel E) Coronal section from the forebrain of a rat 1 year post-ischaemia immunostained for Dcx (in green) and GFAP (in red), showing chains of neuroblasts (indicated by white arrowheads) ectopically directed towards the affected area (delineated by the interrupted line, the star indicates the core of the lesion in the striatum). [Scale bar: in A, B 30 μm; in E 100 μm; *: p < 0.05 using one-way ANOVA, error bars represent SEM].