Fig. 7.

The presence of phagocytotic cells after ischaemia. (Panel A) Triple immunostaining of a coronal section from a rat brain 4 weeks post-ischaemia. Mitotic cells (PH3 +, in green in A1) were increased in the domain of the SEZ directly affected by ischaemia, as outlined by increased expression of GFAP (A2, boxed area) and swelling of blood vessels (A3). (Panel B) High magnification of a section adjacent to that shown in panel A, focusing on the part directly affected by ischaemia (respective to the area boxed in A2). Immunostaining for ED1 and Dcx shows that macrophages are not observed within the SEZ, which is defined by the presence of Dcx + cells. A detail of the area boxed in B2 is presented in C with the limits of the SEZ shown with a white interrupted line. [Scale bar: 450 μm in panel A; 200 μm in panel B; 100 μm in C].