Fig. S1.

Occurrence of proliferating cells and of immature neurons at sites of lesion. Coronal sections taken from sham-operated (A), ischaemic rats at 4 weeks (B, D) and 1 year (C, E) post-injury were either double immunostained for Ki67 and isolectin B-4 (A–C), or for Dcx (D, E). (Panel A) In normal tissue all proliferating cells are located within the SEZ (approximately up to 90 μm away from the ventricular wall). (Panels B, C) After ischaemia, high numbers of Ki67 + cells appear ectopically within the striatal tissue and specifically in the damaged areas, as delineated by the increased IB-4 staining. (Panel D) At 5 weeks post-ischaemia numerous Dcx + cells have accumulated in the striatal tissue at areas proximal to the core of the lesion (at the lower right part of the image), whilst few positive cells are found at the striatal area lying between the SEZ and the lesion. (Panel E) At 1 year, high numbers of Dcx + cells are still observed at the areas of damage, either near the SEZ or deeper in the tissue. [Scale bars: 200 μm in A–C and 100 μm in D, E].