Fig. S7.

Distribution of ED-1 + cells after ischaemia. (Panels A, B) Double immunostainings of coronal sections from a sham-operated rat brain (panel A) and a post-ischaemic rat (1 year after the insult, in panel B), for Dcx + neuroblasts (in green) and ED1 + phagocytotic cells of the immune system (in red). Note that ED1 + activated macrophages and microglia are virtually absent from the SEZ (A1, B1) with the exception of the area at the dorsal–lateral tip of the ventricle that includes the initial fragment of the rostral migratory stream and few cells indicated by arrowheads in B1. Also note the highly activated state of the SEZ (highlighted by the white interrupted lines) in panel B, as evident by the significant increase in the presence of Dcx + immature neurons. Nevertheless, ED1 + cells are largely observed only outside the niche, at the distant wall of the blood vessel, adjacent to the area of lesion. (Panel C) Immunostaining of a coronal section from a post-ischaemic rat (5 weeks after the insult) for ED1 + (in red), again showing the dense presence of ED1 + cells within the affected striatum, in contrast to few positive cells within the adjacent SEZ (highlighted by the white interrupted line). [Scale bars, 200 μm in A and C; 100 μm in B; LV: lateral ventricle, bv: blood vessel].