Table 1. Summary of main glycan structures in three β-glucocerebrosidase enzymes.
CHO, Chinese-hamster ovary; Manx, amount of mannose sugars on glycans; ND, not detected.
| Property | Taliglucerase alfa | Imiglucerase | Velaglucerase alfa |
|---|---|---|---|
| Engineered cells | Carrot cells | CHO cells | human fibrosarcoma cells |
| Amino acid sequence | R 495H EF at N-terminal; DLLVDTM at C-terminal | R495H | Natural human sequence |
| Method used to produce required glycans | Targeting to vacuole to naturally produce pauci-mannose structures | Exoglycosidase digestion (post-production) to expose mannose sugars | Addition of kifunensine to inhibit glycosylation process and produce high mannose structures |
| Exposed mannose | ~100%* | 40–60% | ~100%† |
| Mannose chain length | Man3 | Man3 | Man5–Man9 |
| Additional sugars | Xylose and/or core (α1-3) fucose | GlcNAc/Gal/SA and core (α1-6) fucose | ND |
*All are paucimannose structures.
†All are high mannose structures.