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. Author manuscript; available in PMC: 2013 Sep 25.
Published in final edited form as: Br J Haematol. 2011 Sep 9;155(3):349–361. doi: 10.1111/j.1365-2141.2011.08850.x

Figure 5. Intracellular IFN-γ production and proliferation by CD138 or XBP1-peptide specific CTL in response to myeloma cell lines.

Figure 5

Figure 5

(a) Intracellular IFN-γ production was measured by flow cytometry after stimulation of the respective CD138-CTL, XBP1 US-CTL or XBP1-SP-CTL with HLA-A2+ McCAR or U266 myeloma cell line. Intracellular IFN-γ production was detected in the CTL effector memory (CD3+/CD8+/CD45RO+/CCR7−) subset from each of the respective CTL generated from two individual donors (Donor A, Donor B). A higher level of IFN-γ production was detected in response to McCAR or U266 cells in the CD138-CTL than the XBP1-CTLs. Control T cells did not produce IFN-γ in response to the myeloma cell lines.

(b) Specific cell proliferation of each peptide-specific CTL was evaluated following stimulation with the HLA-A2+ myeloma cell lines on day 5 using a CFSE assay. The percentage of proliferating CTL is shown in the P3 gate (CFSE-low). A higher level of cell proliferation was detected in the CD138-CTL than the XBP1-CTLs in response to McCAR or U266 cells.