Fig. 5.

E. coli Δldt5 DAP auxotroph is impaired for growth on minimal DAP medium. DAP auxotrophic Ldt multiple mutant strains were grown to stationary phase in LB medium supplemented with 200 µg DAP ml⁻¹. Cells were pelleted, washed twice with sterile PBS, and subsequently resuspended in LB medium without DAP. Cell suspensions were plated for viable cells on media containing either 200 µg or 25 µg DAP ml⁻¹. Data were analysed using Student’s t-test. *P values ≤0.05 comparing 200 µg DAP ml⁻¹-treated values with 25 µg DAP ml⁻¹-treated values of the same strain were considered significant. Error bars, ±S.D.