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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: Cell Microbiol. 2012 Nov 6;15(2):213–226. doi: 10.1111/cmi.12041

Figure 6. CHMP2AΔC-CHMP3 polymerization into tubes renders CHMP2A and CHMP3 protease resistant.

Figure 6

(A) Trypsin and proteinase K proteolysis of CHMP2AΔC-CHMP3 tubes. Right panel: lane 1, no trypsin; lanes 2-4 trypsin: CHMP2AΔC-CHMP3 tube ratios of 1:70; 1:35 and 1:20 (w/w), respectively. Right panel: lane 1, no proteinse K; lanes 2-5, proteinase K ratios of 1:500, 1:250, 1:100 and 1:50 (w/w), respectively. (CHMP2AΔC (°); CHMP3 bands produced by trypsin (*) and Proteinase K (Δ) digestion)

(B) Trypsin and proteinase K proteolysis of CHMP3. Right panel: lane 1, no trypsin; lanes 2-4, trypsin: CHMP3 ratios of 1:70, 1:35 and 1:20 (w/w), respectively.

(C) Trypsin and proteinase K proteolysis of MBP-CHMP2AΔC. Lane 1, no trypsin; lanes 2 and 3 trypsin/MBP-CHMP2AΔC ratios of 1:100 and 1:50 (w/w); lanes 4 and 5, proteinase K/MBP-CHMP2AΔC ratios 1:100 and 1:50 (w/w). (MBP bands after proteolysis (*)).