293T cells expressing HIV-1 were treated either with control siRNAs (lanes 1 and 2) or with siRNAs that target the indicated ESCRT-III CHMP family members (lanes 3-8).
(A) Viral titers, n=6 titer measurements from two independent experiments.
(B) Western blot analyses of virion-associated CA and MA proteins released into the cell culture supernatant.
(C) Western blot analyses of intracellular viral Gag derived proteins (panels B and C, anti-MA and anti-CA Abs) and expression levels of cellular GAPDH (loading control)
(D) Western blot analyses of endogenous cellular ESCRT-III CHMP2A, CHMP2B and CHMP3 protein levels, corresponding to the designated lanes of the upper blots.