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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: Cell Microbiol. 2012 Nov 6;15(2):213–226. doi: 10.1111/cmi.12041

Figure 7. ESCRT-III CHMP3 exerts a synergistic effect on ESCRT-III CHMP2A and CHMP2B during HIV-1 budding.

Figure 7

293T cells expressing HIV-1 were treated either with control siRNAs (lanes 1 and 2) or with siRNAs that target the indicated ESCRT-III CHMP family members (lanes 3-8).

(A) Viral titers, n=6 titer measurements from two independent experiments.

(B) Western blot analyses of virion-associated CA and MA proteins released into the cell culture supernatant.

(C) Western blot analyses of intracellular viral Gag derived proteins (panels B and C, anti-MA and anti-CA Abs) and expression levels of cellular GAPDH (loading control)

(D) Western blot analyses of endogenous cellular ESCRT-III CHMP2A, CHMP2B and CHMP3 protein levels, corresponding to the designated lanes of the upper blots.