Figure 1.
Inhibitory effect of cantharidin (CAN) and norcantharidin (NCTD) on the biological behavior of MCF-7 cells. (A) Exposure to various concentrations of CAN and NCTD resulted in dose- and time-dependent growth inhibition. (B) CAN and NCTD treatment inhibited the clone formation ability of MCF-7 cells in a dose-dependent manner. (C) Following CAN and NCTD treatment, the early apoptotic (Annexin V+/PI−) cell populations increased significantly in a dose-dependent manner. (D) CAN and NCTD treatment inhibited MCF-7 cell adhesion in a dose-dependent manner. (E) CAN and NCTD treatment inhibited MCF-7 cell migration in a time-dependent manner. *P<0.05; **P<0.01 indicate significant differences from the respective control groups.