Appendix Table 1.
Symbols for Computation of DNA Yields Equivalent to 5 mL of Whole Blood
| Source | Volume Symbol, mL | Approximate Volume, mL | DNA Yield Symbol, μg |
|---|---|---|---|
| Samples used for fresh and frozen DNA extraction | |||
| Whole blood (tube has fresh whole blood volume = Vtube1) | |||
| White cell (fresh) | Vwbcwb | 1 | Ywbcwb |
| Fresh DNA extraction | Vfrwb | 4 | Yfrwb |
| Frozen DNA extraction | Vfzwb | 4 | Yfzwb |
| All-cell-pellet (tube has fresh whole blood volume = Vtube2) | |||
| White cell (fresh) | Vwbcacp | 1 | Ywbcacp |
| Fresh DNA extraction | Vfracp | 2 | Yfracp |
| Frozen DNA extraction | Vfzacp | 2 | Yfzacp |
| Buffy coat/residual blood cells (tube has fresh whole blood volume = Vtube3) | |||
| For buffy coat white cell (fresh) | Vwbcbc | 0.5 | Ywbcbc |
| For buffy coat fresh DNA extraction | Vfrbc | 1 | Yfrbc |
| For buffy coat frozen DNA extraction | Vfzbc | 1 | Yfzbc |
| Residual blood cells white cell (fresh) | Vwbcrbc | 0.5 | Ywbcrbc |
| Residual blood cells fresh DNA extraction | Vfrrbc | 1 | Yfrrbc |
| Residual blood cells frozen DNA extraction | Vfzrbc | 1 | Yfzrbc |
| Samples used for fresh DNA extraction only | |||
| Whole blood | Vfrwb | 8.5 | Yfrwb |
| All-cell-pellet | Vfracp | 5 | Yfracp |
| Buffy coat | Vfrbc | 1.5 | Yfrbc |
| Residual blood cells | Vfrrbc | 1.5 | Yfrrbc |