Figure 1.

Importance of EGFR as a mediator for other signaling pathways. VSMC of EGFR knockout or wild-type mice were incubated for 5 min with the EGFR ligand EGF (10 µg/l), the PKC activator phorbol-12-myristate-13-acetate (1 µM) or the MR ligand aldosterone (10 nM aldosterone). ERK phosphorylation was assessed by western blot (n = 4–5, data represented as mean ± SEM).