Figure 6.

Importance of full-length MRE1.3 and the SP1-binding site (A) Sequences of MRE1.3, different MRE1.3 deletion constructs and constructs with mutated SP1-binding site are depicted. (B) OK cells transiently transfected with hMR and reporter plasmids were incubated with 10 nM aldosterone or vehicle for 48 h and then reporter gene activities were measured and depicted as percentage of control (MRE1.3 with vehicle). MRE1.3mut1 and MRE1.3mut2, both mutated in the SP1-binding site, were compared with MRE1.3 (N = 3–6, n = 9–18, data represented as mean ± SEM). (C) Transiently hMR- and reporter plasmid transfected OK cells were incubated with 10 nM aldosterone or vehicle for 48 h. Reporter gene activities of four different deletion constructs (MRE1.3b, e and f and MRE1.3mut Δ) were measured and compared with full-length MRE1.3 transactivation (N = 3–6, n = 9–18, data represented as mean ± SEM).