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. 2013 Jul 1;41(17):8308–8318. doi: 10.1093/nar/gkt587

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© The Author(s) 2013. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Dyskerin KD stimulates VEGF-IRES–mediated translation. Transient transfection of DKC1-specific siRNA strongly reduced DKC1 mRNA and protein level in MCF7 and MDA-MB231 cells (A, left and B, left, respectively). [3H]-leucine incorporation indicates that the total protein synthesis is not compromised (A and B, center) after DKC1 KD. IRES-mediated translation was assessed by measuring the FLuc and RLuc activity in MCF-7 and MDA-MB231 (A, right, and B, right, respectively) cells 8 h after the transfection with the bicistronic mRNA transcribed from pRL-VEGF-IRES. siRNA transfection was performed 96 h before cell harvesting. Histograms represent means and SDs from at least three independent experiments. P < 0.05 are considered significant. NS = not significant.