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. 2013 Jul 1;41(17):8308–8318. doi: 10.1093/nar/gkt587

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© The Author(s) 2013. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — DKC1 KD increases VEGF-IRES recruitment to 48S preinitiation complex. (A) Left: representative profile at 260 nM O.D. obtained from MCF7 cytoplasmic extracts: fractions 18–21 were considered to correspond to the small ribosomal subunits. Right: Representative profile of a sucrose density gradient reporting the radioactive intensity per fraction in from DKC1 KD (circles) and control (SCR—triangles) cells extracts, respectively. Peaks of radioactivity coinciding with the identified fractions containing the 48S complexes was generated when MCF7 cytoplasmic extracts were incubated with a [³²P]VEGF IRES mRNA probe. (B) Histogram represent mean and SD of the radioactivity measured in the identified peaks for DKC1 KD and control (SCR) cells extracts. siRNA transfection was performed 96 h before cell harvesting. P < 0.05 is considered significant.