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. 2013 Jul 1;41(17):8182–8195. doi: 10.1093/nar/gkt588

Figure 1.

Figure 1.

DNA strand displacement by PolB1. (A) Schematic representation of the circular and linear primer templates used in PolB1 assays. (B) Strand extension by PolB1. PolB1 (20 nM) was incubated for 15 min at 65°C with P36/C72 or P36/L72 (2 nM) under the standard assay conditions. Products were subjected to electrophoresis in 8% polyacrylamide gel containing 7 M urea in 1× TBE. (C) Sizes of the products of strand displacement by PolB1. PolB1 (20 nM) was incubated at 65°C for various lengths of time with P36/C72 (2 nM). Products were resolved in a 1.2% akaline agarose gel in 50 mM NaOH and 1 mM EDTA. (D) Effect of temperature on strand displacement by PolB1. PolB1 (20 nM) was incubated for 15 min at indicated temperatures with P36/C72 (2 nM). Products were subjected to electrophoresis in 8% polyacrylamide gel containing 7 M urea in 1× TBE. (C) no PolB1 was added. Gels were dried and exposed to radiographic film.