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. 2013 Jul 4;41(17):8094–8106. doi: 10.1093/nar/gkt595

Figure 4.

Figure 4.

Inhibition of DNA methylation restores full induction of CYP1A1 and AhR binding at the CYP1A1 promoter in presence of E2. CYP1A1 (A) and CYP1B1 (B) mRNA levels were quantified in MCF7 cells treated with 10 µM 5-azacytidine for 5 days and grown in estrogen-free medium for 3 days, then treated with 10 nM TCDD or 10 nM TCDD + 100 nM E2 during 24 h. ChIPs of AhR (C) and ERα (D) were performed in MCF7 cells treated with 10 µM 5-azacytidine for 5 days and grown in estrogen-free medium for 3 days, then treated with 10 nM TCDD or 10 nM TCDD + 100 nM E2 for 90 min. Primer B was used for the qPCR analysis.