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Reducing Nctc1 by siRNA does not depress enhancer activity as measured by Igf2 and H19 expression. Nctc1 siRNAs (see Supplementary Table S1 for sequences) reduce steady-state levels of Nctc1 by up to 75% with no effect on Igf2 or H19. RNA levels were analysed by qRT-PCR, normalized for Gapdh levels and then normalized to expression in the absence of siRNA. For each panel, results are depicted as average values with standard deviations from at least three independent transfections.
