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. 2013 Jul 9;41(17):8332–8340. doi: 10.1093/nar/gkt604

Figure 5.

Figure 5.

Requirement of the Ribo-0 regulation of RpoS for the loosely folded inhibitory loop in the 5′ leader. (A) Structure of the rpoS leader and the location where D87 was constructed. The green line denotes the cis-acting antisense element that pairs with the RBS region. The red line denotes Hfq-binding sites. The numbers indicate the nucleotide at the 5′ end of the rpoS leader, relative to the translational start site. (B) Silenced expression of the rpoS-lacZ fusion and low levels of acid resistance in the mutant D87. (C) Predicted secondary structures of the naturally occurring inhibitory loop (upper panel) in the wild-type strain, a perfectly folded inhibitory loop in D87 (middle panel) and an artificial imperfect inhibitory loop in D87im (lower panel). Mutations made in D87im are highlighted in red and the RBS is highlighted in blue. (D) Defect of Ribo-0 in regulation of the rpoS fusion expression and acid resistance in D87, and restoration of the regulation in D87im.