Figure 3.

Characterization of the smallest motif of the ¹⁰C-mediated LDI. Intron 7 sequences of 10- (A), 5- (B) and 3 nt long (C) overlapping deletion mutants are shown with deletions indicated as dashed lines. Nucleotide numbering starts from the beginning of intron 7. Mutants’ names are given on the left. Numbers in mutants’ names represent the positions that were deleted. The effect of F14 and L14 on splicing is indicated similarly as in Figure 2. The region determined to be an interacting partner of ¹⁰C is highlighted. In vivo splicing patterns of the wild-type SMN2 minigene and the representative deletion mutants in the presence of the indicated ASOs are given in the bottom panels. Control ASO was the same as described in Figure 2. Results were analyzed as described in (26).