Figure 4.

Isolation of ncRNA from P. berghei-infected rodent reticulocytes. (A) Bioanalyzer analysis of total RNA extracted from whole blood from uninfected rats (black line) and total RNA extracted from P. berghei schizonts purified from infected rat erythrocytes following lysis of the red blood cells and washing of the schizonts, as described in Supplementary Methods (gray lane). (B) 2D-SEC purification of P. berghei ncRNA. Total RNA from schizonts purified from lysed rodent reticulocytes was resolved on the 2D HPLC system, with Bio SEC-3 300 Å resolution of 5.8S rRNA (1), 5s rRNA (2), putative snRNA/snoRNA (3) and tRNA (4), and Bio SEC-5 1000 Å resolution of the 28s 800 nt rRNA fragment (6) from the co-eluting 18s rRNA and 28s 3000 nt rRNA fragment (5). Individual RNA species were collected from the 2-D HPLC elution, and the purity and identity of each fraction was evaluated by Bioanalyzer analysis as shown in Supplementary Figure S6.