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. 2013 Jul 31;41(17):8266–8279. doi: 10.1093/nar/gkt675

Figure 1.

Figure 1.

Gle2 genetically interacts with factors required for proper pre-60S subunit export. The gle2-1 allele is synthetic lethal (sl, solid line) or synthetic enhanced (se, dashed line) when combined with impaired alleles or deletions of factors required for pre-60S subunit export. gle2-1 allele is sl with xpo1-1, nmd3ΔNES1, bud20Δ, npl3Δ, mex67kraa and mtr2-33 mutants and is strongly se when combined with arx1Δ and ecm1Δ mutants. gle2-1 allele is not sl or se when combined with the indicated yph1-1 and nog1-11 mutants. Strains carrying the WT or mutant alleles were spotted in 10-fold serial dilutions on synthetic defined (SD) plates containing 5-Fluoroorotic Acid (5-FOA, when sl) or yeast extract peptone dextrose (YPD) plates (when se or no interaction) and grown at 25–30°C for 3–5 days. Factors that function in pre-60S nuclear export are indicated in dark gray, pre-60S associated assembly factors are indicated in light gray.