Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Jul 31;41(17):8266–8279. doi: 10.1093/nar/gkt675

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2013. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Figure 6. — Recruitment of Gle2 to late pre-60S subunits requires its basic patch and the GLEBS-interacting surface. (A) Top: gle2-patch and gle2-gis mutant proteins do not co-enrich with export competent pre-60S subunits. TAP purifications using Arx1 as bait were performed in WT (GLE2), gle2Δ, gle2-patch and gle2-gis mutant strains. The calmodulin-sepharose eluates were analyzed on NuPAGE 4–12% gradient gels followed by silver staining and western analyses. The large subunit ribosomal protein L35 served as loading control. Bottom: gle2-patch and gle2-gis mutant proteins are expressed at WT level. The levels of Gle2, gle2-patch and gle2-gis proteins were examined in whole-cell extracts (WCE) by western analysis. (B) The GLEBS-interacting surface of Gle2 is required for anchoring to the NPC. TAP purifications were performed using Nup100 and Nup116 as bait proteins. The tobacco etch viral (TEV) protease treated eluates were examined by western analyses using α-TAP (CBP) and α-Gle2 antibodies.