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. Author manuscript; available in PMC: 2014 Aug 28.
Published in final edited form as: J Am Chem Soc. 2013 Aug 14;135(34):12540–12543. doi: 10.1021/ja4059553

Figure 2.

Figure 2

Evaluation of the activity of Anap specific tRNAC-UAEcLeu/AnapRS in mammalian cells. (A) The construction of pAnap and pEGFP plamids. Identities of different genetic elements used in these constructs are shown. (B) Expression of EGFP-40-Anap in HEK293 cells in the presence (left) and absence (right) of 0.5 mM Anap, achieved by co-transfection of pAnap and pEGFP, analyzed by fluorescence microscopy. (B) ESI-MS analysis of purified EGFP-40-Anap mutant. Expected molecular weight: 29773 Da; observed: 29775 Da.