Skip to main content
. Author manuscript; available in PMC: 2014 Aug 28.
Published in final edited form as: J Am Chem Soc. 2013 Aug 14;135(34):12540–12543. doi: 10.1021/ja4059553

Figure 4.

Figure 4

Subcellular localization of endoplasmic reticulum (ER) and golgi-resident proteins in human cell-lines visualized using Anap (first column, pseudo-colored in blue, superimposed with the bright field image) or mCherry (second column, pseudo-colored in red, superimposed with the bright field image) as fluorescent reporters by confocal microscopy: (A) ER-resident Grp94 (Ser127-Anap)-mCherry (C-terminal) in HeLa cells. (B) ER-resident Grp94 (Leu7-Anap)-mCherry (C-terminal) in HeLa cells. (C) Golgi resident GalT1 (Ser3-Anap)-mCherry (C-terminal) in HEK293 cells. Scale bars: 10 μm.