Figure 5. Janus kinase mediates cytokine-induced disruption of pancreatic epithelial junctions. HPAF-II cells were treated for 48 h with either medium or proinflammatory cytokines with and without pharmacological inhibitors of Janus kinase (JAK). Barrier properties of epithelial cell monolayers were determined by TEER measurement (A), and the integrity of AJs and TJs was determined by immunofluorescence labeling for E-cadherin and ZO-1 respectively (B). A total JAK inhibitor (10 µM) and a selective JAK2 inhibitor (50 µM) significantly attenuate IFNγ/TNFα-driven decrease in TEER and TJ/AJ disassembly (arrows). Data are presented as mean ± SE (n = 3); *p < 0.01; **p < 0.001 compared with cytokine/vehicle-treated cells. Bar, 20 µm.