Figure 6. Cytokines trigger upregulation and membrane translocation of protein kinase D. (A) Representative immunoblots demonstrate an increase in the amounts of phosphorylated (p) protein kinase D (PKD) species in HPAF-II lysates after 24 h exposure to either IFNγ alone or IFNγ plus TNFα. (B) Dual fluorescence immunolabeling of PKD2 (green) and β-Catenin (red) shows a diffuse apical staining of PKD2 in control HPAF-II cells and accumulation of this enzyme at the areas of E-cadherin-based cell-cell contact after 24 h of the IFNγ/TNFα treatment (arrows). Bar, 20 μm.