Figure 7. PKD activity is involved in cytokine-induced disruption of the pancreatic epithelial barrier. HPAF-II cells were treated for 48 h with IFNγ plus TNFα in the presence of either vehicle or two different pharmacological inhibitors of PKD, Gö 6976 (10 µM) and CID755673 (50 µM). Barrier properties of the epithelial monolayers were determined by TEER (A) and fluorescent dextran flux (B) measurements. Both PKD inhibitors significantly attenuate cytokine-induced drop of TEER and increase in transepithelial dextran fluxes. Data are presented as mean ± SE (n = 3); *p < 0.01; **p < 0.001; #p < 0.05 compared with cytokine/vehicle-treated cells.