Table 1.
Residues in huPON1 identified as modulating selectivity and activity by mutagenesis studies, and residues indicated by docking ‘hits’ with OP substrates.
| Residue ID | ||
|---|---|---|
| Experiment | Docking Hits | Location in huPON1 |
| 53 | Calcium Binding Site | |
| 69 | 69 | Periphery of active site |
| 70 | Periphery of active site | |
| 71 | Unresolved Loop | |
| 72 | Unresolved Loop | |
| 74 | Unresolved Loop | |
| 75 | Unresolved Loop | |
| 76 | Unresolved Loop | |
| 79 | Unresolved Loop | |
| 115 | 115 | Near to E53 |
| 168 | Calcium Binding Site | |
| 183 | Near to 168/192 | |
| 190 | Loop over Active Site | |
| 192 | Loop over Active Site | |
| 193 | Loop over Active Site | |
| 196 | Loop over Active Site | |
| 222 | 222 | Periphery of Active site |
| 224 | Calcium Binding Site | |
| 240 | 240 | Periphery of Active site |
| 269 | Calcium Binding Site | |
| 285 | Periphery of Active site | |
| 291 | 291 | Periphery of Active site |
| 292 | 292 | Periphery of Active site |
| 293 | Periphery of Active site | |
| 332 | 332 | Calcium Binding Site |
| 346 | 346 | Periphery of Active site |