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. 2013 Sep 25;8(9):e76038. doi: 10.1371/journal.pone.0076038

Figure 2. Serum deprivation contributes to anti-HIV activity of HD5 in primary CD4+ T cells.

Figure 2

HIV-1 primary isolate 20635–4 (R5 virus, clade C) was incubated with HD5 at different concentrations for 1 h at 37°C. CD4+ T cells from PHA-activated PBLs (A) and PHA-activated CD4+ T cells (B) were incubated with virus-defensin mixture for 2 h at 37°C (no spinoculation) or 1.5 h at 1250×g spinning (spinoculation). After washing off unbound virus, cells were cultured in RPMI containing 10% FBS and IL-2 or 0.3% human AB serum, ITS supplement, and IL-2. HD5 was added back to cell cultures in the presence of IL-2. The level of p24 protein in culture media was measured by p24 ELISA. Data presented are the average ± standard deviation of 3 replicates. Similar results were observed in 3 independent experiments from different donors; *P<0.05, defensin-treated samples vs non-treated controls.