Figure 5. HD5 induces cytotoxicity in primary CD4+ T cells under serum-deprived conditions.

CD4+ T cells (1×10⁴ cells per sample) were treated with HD5 at different concentrations with or without spinoculation. Cells were then cultured in RPMI-1640 containing10% FBS and IL-2 or RPMI-1640 containing 0.3% human AB serum, 1× ITS supplement, and IL-2. HD5 was added back. After overnight culture, cell proliferation was measured by MTS assay (A); cytotoxicity was measured by CytoTox-Glo cytotoxicity kit (B). Data presented are the average ± standard deviation of 3 replicates; *P<0.05, defensin-treated samples vs non-treated controls. Similar results were observed in 3 independent experiments.