Figure 6. HD5 induces cytotoxicity in HIV-exposed primary CD4+ T cells under serum-deprived conditions.

HIV-1_JR-FL was incubated with HD5 at different concentrations at 37°C for 1 h. CD4+ T cells were incubated with defensin-virus mixture with or without spinoculation as described in Fig. 2. Cells were washed and then cultured with the original concentration of HD5 in either RPMI-1640 containing10% FBS and IL-2 or RPMI-1640 containing 0.3% human AB serum, 1× ITS supplement, and IL-2. After overnight culture, cytotoxicity was measured by CytoTox-Glo cytotoxicity kit (Promega). Data presented are the average ± standard deviation of 3 replicates; *P<0.05, defensin-treated samples vs non-treated controls. Similar results were observed in 2 independent experiments.