Table 1. Purity of CD4+ T cells from different isolation protocols.
CD4+ T cells from PHA-activated PBLs (percent) | PHA-activated CD4+ T cells (percent) | |||
1 day | 3 days | |||
Donor 1 | Viable | 45.2 | 97.3 | 75.0 |
CD3+CD4+ | 84.8 | 98.2 | 97.1 | |
CD3+CD4− | 2.5 | 0.2 | 1.4 | |
Donor 2 | Viable | 86.7 | 97.5 | 85.5 |
CD3+CD4+ | 83.5 | 98.0 | 92.3 | |
CD3+CD4− | 9.4 | 0.2 | 5.2 | |
Donor 3 | Viable | 85.6 | 95.9 | 83.2 |
CD3+CD4+ | 82.3 | 98.1 | 96.9 | |
CD3+CD4− | 2.8 | 0.5 | 1.5 |