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. 2013 Sep 25;8(9):e75100. doi: 10.1371/journal.pone.0075100

Figure 8. Divergent PKCµ and PKCβII mRNA expression in pure CD34+ and CD34− orbital fibroblast subsets.

Figure 8

A parental strain of TAO orbital fibroblasts (containing mixed CD34+ and CD34− cells) was sorted into pure CD34+ and CD34− subsets by FACS as described in Experimental Procedures. These were then cultured for 48 h., RNA was isolated, andRT-PCR performed for (left panel) PKCµ and (right panel) PKCβII using the CT method. Ct values were normalized to their respective GAPDH levels. Data are expressed as the mean ± SD of three independent replicates from a single experiment, representative of three performed. PKCµ mRNA was undetectable in CD34+ subsets in all 3 studies while PKCβII mRNA levels were 44±7-fold above their respective parental fibroblast cultures.