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. 2013 Sep 25;8(9):e74777. doi: 10.1371/journal.pone.0074777

Figure 3. SDS-PAGE analysis of His-tagged recombinant tropine forming tropinone reductase of W. somnifera (WsTR-I).

Figure 3

A, induced expression of WsTR-I in E. coli: lane 1, un-induced culture extract; lane 2, induced soluble fraction; lane 3, induced insoluble fraction; lane 4, protein molecular weight marker; B, lane 1, enzyme eluted from affinity Column (Ni++-NTA); lane 2, crude soluble fraction; lane 3, protein molecular weight marker; C, lane 1, protein molecular weight marker; lane 2, crude soluble fraction; lane 3 and 4, enzyme eluted from gel filtration column.