Table 1. Comparative account of catalytic parameters (pH optima and Km) of W. somnifera tropine forming tropinone reductase (WsTR-I) with other plant TRs reported from plants.
Enzyme | pH Optima | Km (mM) | Reference |
WsTR-I* | 5.7 | 1.27 | Present study |
StTR-I* | 6.0 | 0.188 | Kaiser et al. [46] |
StTR-II* | 6.4 | 0.033 | Keiner et al. [57] |
DsTR-I* | 7.0 | 0.117 | Nakajima et al. [41] |
DsTR-II* | 7.0 | 0.048 | Nakajima et al. [41] |
DsTR-I# | 6.4 | 1.3 | Portsteffen et al. [58] |
DsTR-II# | 6.4 | 0.11 | Portsteffen et al. [58] |
HnTR-I* | 5.9 | 1.01 | Hashimoto et al. [35] |
HnTR-II* | 5.9 | 0.034 | Hashimoto et al. [35] |
HnTR-II# | 5.8 | 0.035 | Drager et al. [46] |
CoTR-I* | 8.0 | 3.29 (at pH 6.4) | Brock et al. [48] |
Recombinant protein expressed in E. coli.
Native protein purified from the plant source.