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. 2013 Sep 25;8(9):e74777. doi: 10.1371/journal.pone.0074777

Table 1. Comparative account of catalytic parameters (pH optima and Km) of W. somnifera tropine forming tropinone reductase (WsTR-I) with other plant TRs reported from plants.

Enzyme pH Optima Km (mM) Reference
WsTR-I* 5.7 1.27 Present study
StTR-I* 6.0 0.188 Kaiser et al. [46]
StTR-II* 6.4 0.033 Keiner et al. [57]
DsTR-I* 7.0 0.117 Nakajima et al. [41]
DsTR-II* 7.0 0.048 Nakajima et al. [41]
DsTR-I# 6.4 1.3 Portsteffen et al. [58]
DsTR-II# 6.4 0.11 Portsteffen et al. [58]
HnTR-I* 5.9 1.01 Hashimoto et al. [35]
HnTR-II* 5.9 0.034 Hashimoto et al. [35]
HnTR-II# 5.8 0.035 Drager et al. [46]
CoTR-I* 8.0 3.29 (at pH 6.4) Brock et al. [48]
*

Recombinant protein expressed in E. coli.

#

Native protein purified from the plant source.