Figure 6. Leucine supplementation in already obese mice does not affect body weight, adiposity or energy expenditure.

(A) Body weight curve, (B) mean daily food intake, (C) fat mass and (D) lean mass composition and (E) 24-h energy expenditure in obese mice supplemented or not with leucine in drinking water while maintained HFD (n = 6–7 mice per group). Arrows in (A) indicates when in vivo experiments were carried out. EE: energy expenditure analysis; MRI: magnetic resonance imaging whole-body composition analysis.