Figure 2. Expression of meloe mRNA and MELOE antigens in SW480 line transfected with full-length meloe cDNA.

A. Meloe relative expression measured by RT-qPCR using 3′DR primers on SW480 cells wild type (wt-SW480) or transfected (T-SW480) with full-length meloe cDNA. M113 cells were used as positive control. B. RT-PCR products amplified from RNA of SW480 cells transfected or not with full-length meloe cDNA. Reverse transcriptions were performed on total RNA samples followed by PCR using 5′end and 3′end primers described on Figure 1C. C. TNF secretion by MELOE-1 (M170.48) and MELOE-2 (M170.51) T cell clones in response to SW480 cell line. Co-cultures were performed at 1∶2 clone:SW480 cells ratio and responses were assessed by TNF-α intracellular staining.