Skip to main content
. 2013 Sep 25;8(9):e75233. doi: 10.1371/journal.pone.0075233

Figure 4. Meloe silencing in melanoma cells with a short interfering RNA (siRNA) localized upstream of MELOE-2 ORF.

Figure 4

A. M88 melanoma cells were transfected with 50(control si) or siRNA hybridizing 55 bp upstream of MELOE-2 ORF (meloe si) along with a GFP reporter plasmid. Relative expression levels of meloe mRNA were evaluated on GFP+ sorted cells by qPCR with MELOE-1 primers. B. TNF secretion by MELOE-1 (M170.48) and MELOE-2 (M170.51) T cell clones in response to GFP+ sorted M88 cells transfected with the different siRNAs. Co-cultures with specific clones were performed at 1∶2 clone to melanoma cell ratio and response of each clone was assessed by TNF-α intracellular staining.