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. 2013 Sep 25;8(9):e75460. doi: 10.1371/journal.pone.0075460

Figure 1. Generation of replication-defective adenovirus expressing NP gene of influenza virus (rAd/NP).

Figure 1

(A) A schematic diagram of rAd/NP recombinant genome generated after homologous recombination between the shuttle vector and adenoviral genome. A shuttle vector plasmid containing NP gene of PR8 virus was constructed and recombined with the pAd-Easy vector (Ad5-△E1/E3). (B) Expression of NP in the lysates of HEK293 cells infected with rAd/NP. The expression of NP (indicated by the arrow) was confirmed by immunoblotting assay as described in the Materials and Methods.

B. Humoral immune responses induced by mucosal rAd/NP immunization.