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. 2013 Feb 27;33(9):3799–3814. doi: 10.1523/JNEUROSCI.1930-11.2013

Figure 3.

Figure 3.

Ube3a KD disrupts Golgi morphology. A, Knockdown of endogenous Ube3a in clone 9 cells stably expressing Ube3a shRNA. NT, Nontransfected cells; KD, KD cells stably expressing shRNA against Ube3a; Ctrl, cells stably expressing a nontargeting scrambled shRNA. B, Quantification of Ube3a levels in scrambled control (Ctrl) and Ube3a KD cells, clones KD and KD2, relative to nontransfected (NT) clone 9 cells. **p ≤ 0.001. ns, Not significant (p = 0.30). C, D, Electron micrographs of GA in (C) control cells stably expressing scrambled shRNA and (D) stable Ube3a KD cells clones KD and KD2. The GA in control cells have narrow, elongated cisternae arranged in a characteristic stacked structure (white arrows). In contrast, Golgi cisternae in Ube3a KD cells are swollen (asterisks) and disorganized. Scale bars, 500 nm.